Study on purification and immunologic effect of 42KD protein from group B Streptococcus
- VernacularTitle:B群链球菌42KD蛋白提取及免疫学效果初步探讨
- Author:
Ping WANG
;
Xiangming LI
;
Jianhua WANG
- Publication Type:Journal Article
- Keywords:
Group B Streptococcus;
Conjugate vaccine;
Immunity;
Active protection;
Passive
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):928-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective To look for a vaccine on the base of surface protein which can elicit protective immunity.Methods Purification of 42KD protein from Streptococcus serotype Ⅲ was used one-step chromatography.Antibody response was evaluated in mice immunized by 42KD protein with different immunization dose and by the different immunization procedure.After immunizing the mice in the optimum procedure,we challenge the mice in intradermal with Streptococcus to observe the active and passive protection.Results The molecular weight(Mr)of the protein was 42.33×103 and its point isoelectric(pI)was 6.6.Its property might be a glycoprotein which could be released to supernatant.The highest titer of antibody against 42KD protein Was 1:24576.Which indicated that 42KD protein had good immunogenicity.The immunity exponent of NIH mice was 100 in test group and infant mice was 40.Conclusion The data suggests that 42KD protein may elicit protective immunity to adult and infant mice.Through protecting test with different immunizing doses,the findings indicate that protecting capability is relative to the titer of IgG specificantibody.
