The expression of IL-1β and MMP-1 in the knee cartilage on experimental osteoarthritis rabbit
- VernacularTitle:IL-1β、MMP-1在兔膝关节骨性关节炎模型软骨中的表达
- Author:
Lin ZOU
;
Changjie ZHANG
;
Ying KONG
;
Gang WU
- Publication Type:Journal Article
- Keywords:
Osteoarthritis;
IL-1β;
MMP-1
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(9):584-588
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model of knee osteoarthritis (OA) , and to explore the relationship between cartilage degeneration and the expression of IL-1β and MMP-1. Methods A total of 16 healthy male rabbits (32 hind knee joints) were used. The experimental OA model was established in their left knees by intraarticular injection of0.2 ml of 4% papain solution and 0.1 ml of 0.03 mol/L L-cysteine solution. The right knees were used as the controls and were treated with intraarticular injection of 0.3 ml of normal saline solution. General and histological scoring, and IL-1β and MMP-1 expression intensity were compared between the 2 groups. Results The degree of cartilage degeneration in the left knees was higher than in the right knees as observed by naked eye and using an electron microscope. The left knees scored significantly higher than the right ones with Mankin's scoring system. There was significantly greater expression of IL-1β and MMP-1 in the chondrocytes of the left knees. Conclusions lntraarticular injection of papain and L-cysteine can induce experimental knee osteoarthritis in rabbits. The expression of IL-1β and MMP-I is significantly related with carti-lage degeneration.
