The proportion changes of CD4+CD25+ regulatory T cells in the periphery blood of patients with Vogt-Koyanagi-Harada disease
- VernacularTitle:伏格特-小柳-原田综合征患者外周血中CD4+CD25+调节性T细胞的比例变化
- Author:
Keyan WANG
;
Jiahua Lü
;
Gezhi XU
- Publication Type:Journal Article
- Keywords:
Uveitis/physiopathology;
Uveomeningoencephalitic syndrome/physiopathology;
T-Lymphocytes,regulatory/immunology;
Flow cytometry
- From:
Chinese Journal of Ocular Fundus Diseases
2008;24(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the proportion changes of CD4+CD25+FOXP3+ T cells in peripheral blood of patients with Vogt-Koyanagi-Harada disease(VKH)before and after one month of treatment. Methods The peripheral blood samples from 15 patients with VKH disease before and after one month of treatment by glucocorticoid,and from 15 healthy volunteers were collected,and lymphoeytes were separated from them.CD4+CD25+regulatory T cells were Iabeled by antibodies of cell surface marker CD4、CD25 and transcription factor FOXP3.The proportion of CD4+CD25+FOXP3+ T cells were detected by flow cytometry. Results Before the treatment,the percentage of CD4+ CD25+FOXP3+ T cells in periphery blood was(0.30±0.19)%of CD4+ cell in VKH patients,and(1.41±0.52)%in control group,the difference was statistically significant(t=7.665,P＜0.01);after one month of treatment,the VKH patients group was(1.28±0.54)%which close to the control group.However there were two patients whose CD4+ CD25+ T cells inereased extraordinarily after one month of treatment. Conclusions The proportion of CD4+ CD25+ FOCP3+ T cells in periphery blood in VKH patients were lower than control group obviously before treatment,but were close to eontrol group after treatment.Those results indicated that VKH diseases may be associated with the decreased proportion of CD4+ CD25+ regulatory T cells.
