Effects of internal and external biliary drainage on inducible nitric oxide synthase mRNA expression of Kupffer cell and serum tumor necrosis factor-alpha in rats with obstructive jaundice
- VernacularTitle:胆汁内、外引流术对梗阻性黄疸大鼠血清肿瘤坏死因子和肝脏库普弗细胞诱导型一氧化氮合酶表达的影响
- Author:
Ying MENG
;
Yunsheng YANG
;
Wen LI
- Publication Type:Journal Article
- Keywords:
Jaundice,obstructive;
Drainage;
Kupffer cell;
Nitric oxide synthase Tumor necrosis factor
- From:
Chinese Journal of Digestion
2008;28(10):669-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of internal and external biliary drainage on inducible nitric oxide synthase (iNOS) mRNA expression of Kupffer cells and serum tumor necrosis factor-alpha (TNF-α) in rats with obstructive jaundice. Methods Forty-eight male Sprague-Dawley rats were randomly assigned to obstructive jaundice (OJ), sham operation (SH), internal biliary drainage (ID) and external biliary drainage (ED) groups with 12 each. Kupffer cells were isolated by in situ hepatic perfusion and digestion with collagenase type Ⅳ, and purified by cell culture attachment. The expression of iNOS mRNA of Kupffer cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and the serum TNF-α concentration was measured using ELISA method. Results The serum TNF-α level was increased in OJ group[ (110.84±26.3) pg/ml ]compared with SH group [-(88.4±17.9) pg/ml, (P=0.045)]. The TNF-α level in ID group[ (89.8±28. 3) pg/ml ]was significantly lower than that in ED group[ (118.64±22.7) pg/ml, (P=0. 011) ]and OJ group (P=0. 059). Expression of iNOS mRNA of Kupffer cells was significantly higher in OJ group (0. 824± 0. 24) compared with SH group (0. 384±0.35,P=0. 005). After relieving the OJ, the iNOS mRNA expression in ED group (0. 974± 0.48) was not suppressed (P=0. 321). On the contrary, the iNOS mRNA expression in ID group was suppressed and significantly lower than that in ED group (0. 59±0. 35) (P=0. 016). Conclusions Internal biliary drainage is superior to external drainage in terms of reversing the elevated serum TNF-α and in suppressing the iNOS mRNA expression of Kupffer cells in rats with obstructive jaundice.
