Impact of interleukin 1β on the expression of lectin-like oxidized LDL receptor 1 and ABCA1 in human mesangial cell line
- VernacularTitle:白细胞介素1β对人肾小球系膜细胞LOX-1和ABCA1表达的影响
- Author:
Hua LIU
;
Hang LI
;
Jianling TAO
;
Yubing WEN
;
Xuewang LI
- Publication Type:Journal Article
- Keywords:
Interleukin 1;
Receptors,LDL;
Cholesterol;
Mesangial cell;
Leclin-like oxidized LDL receptor 1;
ATP-binding cassette transporter A1
- From:
Chinese Journal of Nephrology
2008;24(10):725-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the impact of IL-1β on the expression of lectin-like oxidized LDL receptor 1 (LOX-1) and ATP-binding cassette transporter A1 (ABCA1) in human mesangial cell line (HMCL), and its association with cholesterol homeostasis of HMCL. Methods Levels of LOX-1 and ABCA1 of HMCL induced by IL-1β were examined by using real-time PCR and Western blot. Results IL-1β up-regulated LOX-1 mRNA and protein expression. Treated with 5 μg/L IL-1β, the levels of LOX-1 mRNA and protein reached the peak after 6 h and 24 h of stimulation and were 6.87 folds and 1.88 folds of control rspectively. The expression of ABCA1 mRNA and protein of lipid-loaded HMCL was down-regulated by IL-1β Stimulated with 5 μg/L IL-1β the expression of ABCA1 mRNA and protein decreased to the lowest level, 19.0% and 50.62% of the baseline respectively. Conclusions The expression of LOX-1 can be up-regulated while the expression of ABCA1 can be decreased by the stimulation of IL-1β. IL-1β can enhance dyslipidemia and influence the balance of cholesterol homeostasis of HMCL.
