Protective effects of fluvastatin on cyclosporine A-induced nephrotoxicity in rat fibroblast
- VernacularTitle:氟伐他汀对环孢素A所致大鼠成纤维细胞肾毒性的保护作用
- Author:
Genyang CHENG
;
Zhangsuo LIU
;
Shuzhen LIU
;
Hong LI
;
Jiansheng LI
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Fibroblasts;
Cell proliferation;
Transforming growthfactor β;
Fibronectins;
Fluvastatin
- From:
Chinese Journal of Nephrology
2008;24(9):647-650
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of fluvastatin(Flu) on rat renal fibroblasts proliferation and cytokines expression induced by cyclosperine A (CsA). Methods The fibroblasts were cultured with CsA or with CsA plus fluvastatin. The cellular proliferation was determined by MTT colorimetry. The mRNA expression of transforming growth factor β1(TGF-[β1), connetive tissue growth factor (CTGF) and c-fos was detected by BT-RCB. The protein level of flbronecfin (FN) was measured by Western blotting. Results CsA inhibited the proliferation of fibroblasts in dose- and time-dependent manner (P＜0.05). Treatment with Flu accelerated the suppression of fibroblasts resulted from CsA (P＜0.O1). CsA stimulated the expression of TGF-β1, CTGF, c-fos and FN compared with control group(P＜0.05), which could be down-regulated by Flu (P＜0.05). Conclusion Fluvastatin may relieve CsA-induced nephrotoxicity in rat fibroblasts.
