Influence of COX-2 inhibitor on expression of Ang-1 and Ang-2 protein in human tongue squamous cell line Tca-8113
- VernacularTitle:选择性环氧合酶-2抑制剂对人类舌鳞癌Tca-8113细胞血管生成素蛋白表达的影响
- Author:
Yunxia WU
- Publication Type:Journal Article
- Keywords:
Prostaglandin-Endoperofide synthases;
Cycloaxygenase inhibitors;
Angiopoietin-1;
Angiopoietin-2;
Tongue neoplasms;
Carcinoma,squamous cell
- From:
Cancer Research and Clinic
2008;20(9):584-587
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the influence of Nimesulide(NIM),a selective Cyclooxygenase-2 (COX-2) inhibitor, on the expression of Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) protein in human tongue squamous cell carcinoma Tca-8113 cell lines. Methods Tca-8113 cell were cultured in vitro, and exposed to a range of concentrations of nimesulide (NIM) (0, 25, 50, 100, 200 μmol/L) for 24 h, 48 h, 72 h, and the PGE2 level in the cells cultured supernate was quantitated by the radioimmunoassay, and the expression of Ang-1 and Ang-2 protein in human tongue squamous cell line Tca-8113 were measured by the SABC immunocytochemistry staining simultaneously. Results The PGE2 level in the cultured supematant and expression of Ang-2 in Tca-8113 cells decreased by NIM in a concentration and time dependent manner, but not Ang-1. Conclusion NIM can decrease PGE2 level and Ang-2 expression, which may be involved in the inhibition of cancer angiogenesis of COX-2 dependence mechanisms.
