Effect of exercise on gene expression of calcium modulin in myocardial sarcoplasmic reticulum of diabetic rat
- VernacularTitle:运动对1型糖尿病大鼠心肌肌浆网钙调控蛋白表达的影响
- Author:
Weihua JIANG
;
Daya LUO
;
Lehan YU
;
Rong DUAN
;
Fusheng WAN
- Publication Type:Journal Article
- Keywords:
Exercise;
Diabetes;
Calcium modulin;
Sarcoplasmic reticulum;
Phaspholamban;
Ry-anodine receptor type 2
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2008;30(10):653-657
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of exercise on calcium modulin in myocardial sarcoplasmic re-ticulum of animal type 1 diabetes model in rat. Methods A total of 40 Spragne-Dawley rats were randomly dividedinto 4 groups : a normal control group, an exercise training group, a diabetes group and a diabetes plus exercise-traininggroup. At the end of 4- week-exercise training after the establishment of the diabetes model by intraperitoncal injectionof sterptozotocin, the animals were sacrificed and the level of blood glucose, insulin, blood fat and glycosylated serumprotein were tested. The gene expression of calcium modulin proteins was measured by reverse transcription-polymerasechain reaction, and the Western blotting technique was used to measure the protein of sarcoplasmic endoplasmic reticu-lure Ca<'2+> -ATPase (SERCA2) and phaspholamban (PLB). Results The level of biochemical indicator of exercisegroup is not affected when comparing with that of the control group, but significantly changed in diabetic group ( P <0. 01 ) ; The level of blood glucose, insulin, blood fat and glycosylated serum protein were ameliorated in diabetic rats inthe exercise training group. No significant changes in mRNA level of SERCA2, PLB and ryanodine receptor type 2(RYR2) were observed between control and diabetic group, the same to protein expression of SERCA2 and PLB. Butexpression of calcium modulin mRNA was significantly increased in exercise group and diabetic rats in the exercisetraining group comparing with that of the control and diabetic groups ( P < 0.01 ), the same to protein expression ofSERCA2 and PLB. Conclusion Exercise exerted good protective effects on the myocardial injury with 1 type diabetesrat, which might attribute to the upregnlated expression of SERCA2, PLB and RYR2 in diabetic rat heart.
