Evaluation of multiple locus variable number tandem repeat analysis (15-locus set) in genotyping of Mycobacterium tuberculosis strains of the Beijing genotype
- VernacularTitle:多位点数目可变串联重复序列分析在北京基因型结核分枝杆菌基因分型中的应用
- Author:
Weiwei JIAO
;
Zhaona LI
;
Lin SUN
;
Guizhi SUN
;
Mokrousov IGOR
;
Yajie GUO
;
Narvskaya OLGA
;
Adong SHEN
- Publication Type:Journal Article
- Keywords:
Myeobacterium tuberculosis;
Minisatellite repeats;
Genotype;
Polymorphism,restriction fragment length
- From:
Chinese Journal of Laboratory Medicine
2008;31(11):1249-1252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of multiple locus variable number tandem repeat analysis (MLVA, 15-locus set)for genotyping of Mycobacterium tuberculosis (MTB) strains of Beijing genotype. Methods Total 72 Beijing genotype MTB strains obtained from Beijing Thoracic Hospital were genotyped by MLVA (15-locus set). The results were compared with that generated from "gold standard"IS6110-RFLP. Results After genotyped by MLVA ( 15-locus set), 72 strains were grouped into 59 types,of which 53 were unique types. The Hunter-Gaston index (HGI) of MLVA ( 15-locus set) was 0.990. The loci QUB-11b, Mtub 21 and QUB-26 were polymorphic in selected Beijing genotype strains. Genotyping by IS6110-RFLP generated 69 types, of which 66 were unique types. The HGI of IS6110-RFLP was up to 0.999, and the MLVA (15-locus set) clustered strains could be further subdivided. Conclusion MLVA(15-locus set) showed better discriminatory ability in Beijing genotype MTB strains, though secondary typing of clustered strains by IS6110-RFLP is needed.
