The effect on activation of T lymphocytes with SLE by blocking 4-1BB/4-1BBL pathway
- VernacularTitle:阻断4-1BB/4-1BBL通路在系统性红斑狼疮患者T淋巴细胞活化中的作用
- Author:
Rong ZHANG
;
Yun GUO
;
Lijuan ZHAO
;
Weiguo XIAO
;
Yi JIANG
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythematosus;
T lymphocyte;
4-1BB;
p38 MAPK;
NF-kB
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):891-894
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of costimulatory molecules 4-1BB/4-1BBL on T cell activation with systemic lupus erythematosus(SLE)patients.Methods The expression of NF-kB mRNA and p38 MAPK mRNA of T cells with 20 SLE patients and 20 normal controls before activation,after activation and blocked bv 4-1BB/4-1BBL were detected bv RT-PCR.The protein levels of p-p38 MAPK and NF-kB were detected by Western blot.Results The expression of NF-kB mRNA and p38 MAPK mRNA and the protein expression of NF-kB and p-p38 MAPK of T cell with SLE patients were higher than that of normal controls(P＜0.01).There were more expression of NF-kB mRNA,p38 MAPK mRNA,NF-kB protein and p-p38 MAPK protein of T cell with SLE patients stimulated by anti-CD3 monoantibody(P＜0.01).In anti-4-1BB monoantibody blockage group,there were decreased expression of p38 MAPK mRNA and p-p38 MAPK protein(P＜0.01).but not the decreased NF-kB mRNA and protein expression.Conclusion Costimulatory molecule 4-1BB plays a critical role in T cell activation of SLE by p38 MAPK signal pathway.
