Comparative evaluation of eight third generation and four fourth generation anti-HIV diagnostic reagents
- VernacularTitle:8种第3代和4种第4代人免疫缺陷病毒抗体诊断试剂的比较
- Author:
Xiuhua LI
;
Sihong XU
;
Aijing SONG
;
Jianhui NIE
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
HIV;
Diagnostic reagents;
Genotype;
Seroconversion panel
- From:
Chinese Journal of Microbiology and Immunology
2008;28(10):955-958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the differences between the third and the fourth generations of anti-HIV assays,and different kits within the same generation.Methods A total of 989 HIV-negative samples,185 samples positive for HIV-1 RNA.1st-generation international references of HIV antibodies and samples from 9 sets of BBI seroconversion panels were detected by 8 kits of the third generation and 4 kits of the fourth.Results The fourth generation kits can detect HIV infection earlier than the third generation kits.However,the detected days of HIV infection with different kits of the fourth generation were different whilst no significant difierences were found with difierent kits of the third generation.Furthermore,the capacity of detecting samples with different genotypes for different reagents was different,especially the capacity of domestic reagents on detecting HIV-1 O group and HIV-2 samples was relatively weak.Conclusion These data provided information to improve the quality of anti-HIV diagnostic reagents further.
