The expressions of nuclear factor-κB and activator protein-1 in oxazolone induced colitis in mice
- VernacularTitle:核因子-κB和激活蛋白-1在恶唑酮诱导的小鼠实验性肠炎中的表达
- Author:
Yunmin LU
;
Weixiong CHEN
;
Jinlian CHEN
;
Jinshui ZHU
;
Niwei CHEN
- Publication Type:Journal Article
- Keywords:
Ulcerative colitis;
Nuclear factor-κB;
Activator proten-1;
Oxazolone
- From:
Chinese Journal of Digestion
2008;28(10):682-685
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of nuclear factor(NF)-κB and activator protein (AP)-1 in oxazolone induced colitis in mice and their mechanisms. Methods Twenty-four mice were randomly divided into normal group and model group with 12 each. Experimental colitis was induced with skin sensitization of 3% oxazolone for 5 days, then rectal administration of 0.15 ml of 0. 5% oxazolone solution in mice. All mice were sacrificed on day 3. Peripheral blood mononuclear cells (PBMC), spleen mononuclear cells (SMC) and lamina propria mononuclear cells (LPMC) were isolated from the colon tissues. Expression of NF-κB and AP-1 in SMC, LPMC and PBMC were determined by fluorescence quantitative polymerase chain reaction (PCR). The colitis was evaluated histologically. Results The expressions of NF-κB and AP-1 in SMC, LPMC,PBMC of model groupwere significantly higher than those in normal group(NF-κB : 5.62±0.78 vs. 3.16±0.59,5.46±0.38 vs. 3.18±0.58, 5.65±0.56 vs. 3.36±0.59, P＜0.01; AP-1; 5.61±0.54 vs. 3.22±0.50, 5.50±0.69 vs. 3.19± 0.40,5.67±0.44 vs. 3. 27±0.41, P＜0.01). Conclusion The activation of NF-κB and AP-1 are involved in the mechanisms of ulcerative colitis.
