The protective effect of vasoactive intestinal peptide on gut barrier in severe acute pancrititis in rats
- VernacularTitle:血管活性肠肽对重症急性胰腺炎肠屏障保护作用的实验研究
- Author:
Zhongkai LU
;
Weichang CHEN
- Publication Type:Journal Article
- Keywords:
Vasoactive intestinal peptide;
Toll-like receptor 4:Pancreatitis;
Gat barrier
- From:
Chinese Journal of Digestion
2008;28(11):746-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of vasoactive intestinal peptide(VIP) on gut barrier in rats with severe acute pancrititis(SAP)and its mechanisms.Methods Fifty-four SD rats were randomly divided into sham operated(So)group,SAP group and VIP group.The model of SAP was induced by retrograde injection of 4%sodium taurocholate into the bili-pancreatic duct.The rats in VIP group were intraperitoneal injected with VIP of 5×10-9 nmol 5 minutes after model established.The endotoxin in plasma was measured at 1,6 and 1 2 hour.The expression of Toll-like receptor(TLR)4 in gut mucosa was examined using RT-PCR and immunohistochemistry and mucosa tissue were detected using electroscopic examination.Results The concentration of endotoxin in plasma and expression of TLR4 were both increased at 1 hour and reached peak at 1 2 hour in SAP group compared with SO group(no expression of TLR4),but were decreased in VIP group.A correlation was found between endot6xin and expression of TLR4.The electroscopic examination showed that the pathological injury in intestine was severe in SAP group than that in VIP group.Conclusion The protective effect of VIP in gut barrier function may contribute tO down-regulation of TLR4 expression in gut mucosa.
