Construction of multiple antigenic peptide and the immunity analysis
- VernacularTitle:钩端螺旋体多抗原肽的构建及免疫性分析
- Author:
Xuai LIN
;
Yujuan SUI
;
Yafei MAO
;
Jie YAN
- Publication Type:Journal Article
- Keywords:
Leptospirosis;
Multiple antigenic peptide;
Expression;
Immunocompetence
- From:
Chinese Journal of Microbiology and Immunology
2008;28(11):1010-1013
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the multiple antigenic peptide (MAP) gene and E. coli ex-pression system, based on the out membrane protein OmpL1, LipL21 and LipL32 from Leptospira interro-gans, and better understanding of the immunological activity of the recombinant protein. Methods Using MI3KE display and Western blot, the advantage epitopes of OmpL1, LipL21 and LipL32 were identified and used to synthesize a new gene, then its prokaryotic expression system was constructed. The expression of re-combinant protein was determined by SDS-PAGE. The immunity activity of the recombinant protein was iden-tified by Western blot and ELISA. Results The synthetic gene was effectively expressed in E. coli and mainly presented in soluble form. The expression protein could react with the antileptospirosis antibodies in rabbit and human sera, which contained different serogroups. Conclusion The recombinant MAP gene of leptospires was successfully constructedand and expressed in E. coli. The recombinant protein had a good immune activity, and could cross-reacted with antileptospirosis antibodies from different serogroups.
