Preparation and immunological properties of hepatitis B virus surface antigen-tetanus toxoid conjugate vaccine
- VernacularTitle:乙型肝炎表面抗原-破伤风类毒素结合疫苗的制备及免疫学特性
- Author:
Lunbiao CUI
;
Zhongyu HU
;
Yiyue GE
;
Xiangjun ZAI
;
Zhiyang SHI
;
Yongjun JIAO
;
Yuhua QI
;
Zhenglun LIANG
;
Fengxiang LI
;
Hua WANG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus surface antigen;
Tetanus toxoid;
Conjugate vaccine;
Immune re-sponse
- From:
Chinese Journal of Microbiology and Immunology
2008;28(11):1016-1019
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare and study the immunogenicity of hepatitis B virus surface anti-gen (HBsAg)-tetanus toxoid (TT) conjugate vaccine. Methods Tr was activated by cyangen bromide and reacted with adipic acid dihydrazide, then HBsAg-TT conjugate was prepared by carbediimide. Conjugate, HBsAg or hepatitis B vaccine was injected subcutaneously into mice. Anti-HBsAg and HBsAg-specific T cell response elicited by these immunogens were assayed. Results New HBsAg-TT conjugate elicited higher levels of anti-HBsAg and HBsAg positive conversion rates after the immunization than did HBsAg alone or hepatitis B vaccine. Conjugate induced mesdy antibodies of the IgG2a subclass, while HBsAg alone or hepa-titis B vaccine mainly elicited anti-HBsAg in the IgG1 subclass. The number of IFN-γand IL-2 secreting T cells induced by conjugate was also significantly higher than that did by HBsAg or hepatitis B vaccine. Con-clusion This study indicated new HBsAg-TT conjugate can induce both stronger humoral and TH1 type of cellular immune response.
