Effects of EEFIA2 gene on the invasion and migration of pancreatic neoplasms
- VernacularTitle:人类真核延伸因子1A2对胰腺癌细胞侵袭、转移能力的影响
- Author:
Qi ZHU
;
Haixia CAO
;
Jia HUANG
;
Jianqing DING
;
Su ZHANG
;
Baiwen LI
;
Weiyan YAO
;
Yongping ZHANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
EEF1A2 protein,human;
Cell lines,tumor
- From:
Chinese Journal of Digestion
2008;28(11):751-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the change of invasion and migration of the pancreatic carcinoma cell line SW1990 transfeeted with EEF1A2 gene.Methods Pancreatic carcinoma cell line SW1990 was transfected with EEF1A2 by recombinant adenovirus vector.The alteration of motility、invasion and adhesion property of SW1990 was evaluated by wound healing assay,transwell With or without Matrigel basement membrane and adhesion assay.Results Wound healing assay revealed that EEF1A2 enhanced cell motility and transwell assay with Matrigel indicated that the average numbers of transwell cells with EEFlA2 was increased from 23.25±5.23 to 65.42±8.24(P＜0.05).The adhesive rate was substantially increased in EEF1A2 transfected SW1990 cells compared with control cells.Conclusions EEF1A2 gene can promote the migration.invasion and adhesion ability of pancreatic cancer cell in vitro.It is indicated that EEF1A2 may involve in the development of human pancreatic cancer by influencing cell biological characteristics.
