Application of immunoassay technique in clinical laboratory and some problems should being considered
- VernacularTitle:免疫测定技术的临床应用及应注意的问题
- Author:
Jinming LI
- Publication Type:Journal Article
- Keywords:
Immanoassay;
Internal quality control;
Standardization
- From:
Chinese Journal of Laboratory Medicine
2008;31(11):1205-1208
- CountryChina
- Language:Chinese
-
Abstract:
The serological markers of infectious diseases, tumor markers, hormone, serum specific proteins and autoantibodies are tested by immunological methods in clinical laboratories. The immunological methods involved in classic immune agglutination assay, immune precipitation assay and label immunoaszay for trace antigen and antibody detection (such as enzyme-linked immunosorbent assay, chemiluminescence immunoassay and time resolved fluoroimmunoassay). Some of the new immunoassay such as protein chip and immune sensor also tried to be used in clinical laboratories, but it is important that these methods shall be evaluated before being used routine tests. Measurement traceability for quantitative immunoassay using international reference materials and standardization of reagent kit, methods and routine procedures are prerequisites to assure the results uniformity of different laboratories, reagent kits and methods. The carry out, analysis and evaluation of internal quality control are the sources of quality continued improvement of routine tests.
