Viral load and neutralizing activity of plasma from HIV-1 chronic infected former blood donors in Anhui province, China
- VernacularTitle:慢性HIV-1感染人群血浆不同中和能力及与有关因素的研究
- Author:
Yan JI
;
Liying MA
;
Shuiling OU
;
Lin YUAN
;
Jianping SUN
;
Pengfei MA
;
Quanbi ZHAO
;
Yang HUANG
;
Jianghong HUANG
;
Yiming SHAO
- Publication Type:Journal Article
- Keywords:
HIV-1B';
Chronic infection;
Neutralization: Viral load;
CD4
- From:
Chinese Journal of Microbiology and Immunology
2008;28(9):787-790
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association among viral load,CD4 count and neutralizing ac-tivity of plasma from chronic HIV-1 infected former blood donors in Anhui province, China. Methods 294 chronically HIV-1 B' infected individuals from former blood donors in Anhni province were enrolled, whose plasma and peripheral blood mononuelnar cells (PBMC) were isolated. The viral load and CD4 were detec-ted, and the neutralization activity of plasma against primary virus (1597) and lab-adapted strain (SF33) was detected by Luciferase Assay System based on TZM cell line, and 50% neutralizing level was calculated by the Reed-Mueneh method. Results Neutralizing activity responding against SF33 strain was higher than 1597 (P<0.05). There was a positive correlation between neutralizing concentration of plasma against SF33 and viral load (1g) (r = 0.191, P = 0.001), but there was no correlation between neutralizing concen-tration of plasma against 1597 and viral load(lg) (r = 0.097, P = 0.096). Conchtsion In chronic HIV-1 infectious people, neutralizing level against SF33 increases with viral replication, however, there is no asso-ciation of plasma neutralizing against 1597 replication with viral load.
