The establishment of the mouse model based on the pseudovirious of humnn papillomavirus types 16,45 and 58
- VernacularTitle:人乳头状瘤病毒16、45和58型假病毒小鼠感染模型的建立
- Author:
Xueling WU
;
Hongwei HE
;
Chuntao XHANG
;
Jianhui NIE
;
Jinping FAN
;
Rongguang SHAO
;
Youchun WANG
- Publication Type:Journal Article
- Keywords:
Human papillomavirus (HPV);
Pseudovirus;
Infection model;
Nonoxynol-9
- From:
Chinese Journal of Microbiology and Immunology
2008;28(9):791-794
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the mouse model for human papillomavirus types 16, 45 and 58 by the corresponding pseudovirions. Methods The 293FT cells were co-transfected with eodon-modified HPV eapsids genes together with a reporter plasmid containing the luciferase gene. The cells were collected and lysed, then the pseudovirus was collected and the titration was performed. The mouse was subcutaneous-ly injected with Depo-Provera. After 4 d and intravaginally injected with nonoxynol-9, and 6 h later pseud-oviruses were inoculated in intravaginal. After 7 d, the mouse was instilled luciferin substrate intravaginally, and the expression level of the lueiferase gene was detected by the in vivo Imaging System (IVIS). Results Three types (HPV16, HPV45 and HPV58) of pseudoviruses had been produced and the titer was 3.7×108 TU/ml, 1.5×108 TU/ml and 1.2×108 TU/ml, respectively. The luminescent regions could be detected in the mice which were infected with the pseudovirions, the luminescent signal intensity for types 16,45 and 58 was 1.779×106p/s, 5.738×105×p/s and 1.829×106p/s, respectively. Conclusion The mouse models for HPV16, 45 and 58 have been successfully established based on pseudovirions, which will be very useful for the research of HPV infection intervention, the evaluation of HPV vaccines and the screening of the prophylactic agents.
