Vector construction and expression of soluble mPDL1-hIgGFc and its effect on the proliferation and apoptosis of cells in vitro
- VernacularTitle:可溶性mPDL1-hIgGFc表达载体构建、表达及对诱导细胞增殖与凋亡的影响
- Author:
Jing YANG
;
Wenjun LIAO
;
Guohua WANG
;
Fengrong HE
;
Huifen ZHU
;
Hong DAI
;
Wei ZHOU
;
Xiongwen WU
;
Jinyuan ZHANG
;
Guanxin SHEN
- Publication Type:Journal Article
- Keywords:
mouse PDLI;
Mixed lymphocyte culture;
Proliferation;
Apoptosis
- From:
Chinese Journal of Microbiology and Immunology
2008;28(9):795-798
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct vector expressing soluble mPDL1-hIgGFc and study its effect on the proliferation and apeptosis of cells in vitro. Methods The extrncellular domain of mPDL1 gene was amplified from pmPDL1 vector by PCR and inserted into phIgGFc vector. The recombinant pmPDL1-hIgGFc was transfected into CHO cells by LipofectAMINETM2000, and the transfected cells were named as CHOp. The expression of mPDL1-hIgGFc in the culture supernatants of CHOp was assayed by ELISA and Western blot. The effects of CHOp culture supernatants on mixed lymphocyte culture(MLC) was analysed by Flowm-etry. Results The extracellular domain of mPDL1 gene were obtained from PCR. DNA sequencing and the identification of digestion by HindⅢ and KpnⅠ indicated the recombinant plasmid pmPDL1-hIgGFc was suc-cessfully constructed. ELISA and Western blot analysis proved that the CHOp could express mPDL1-hIgG-Fc. CHOp culture supernatants could inhibit lymphocyte proliferation and induce the apoptosis of the activa-ted T cells in MLC in vitro in a dose-dependent manner. Conclusion The mPDL1-hIgGFc protein could in-hibit lymphocyte proliferation and induce the apoptosis of the activated T cells.
