Therapeutic effects of Ag85A plasmid DNA vaccines in a mouse model of multi-drug resistant Mycobacterium tuberculosis infection
- VernacularTitle:结核分枝杆菌Ag85A质粒DNA疫苗治疗小鼠耐药结核病的效果研究
- Author:
Yan LIANG
;
Xueqiong WU
;
Junxian ZHANG
;
Yourong YANG
;
Ning LI
;
Qi YU
;
Jingying SONG
;
Xuejuan BAI
;
Chenglong LIU
;
Zhongming LI
;
Lan WANG
;
Yingchang SHI
- Publication Type:Journal Article
- Keywords:
DNA vaccine;
Mycobacterium tuberculosis
- From:
Chinese Journal of Microbiology and Immunology
2008;28(9):818-821
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effects of Ag85A plasmid DNA vaccines in a mouse model of multi-drug resistant-(MDR-) Mycobacterium tuberculosis infection. Methods BALB/c mice were infected with Mycobacterium tuberculosis clinical strain HB361 with isoniazid and rifampin resist-ance by intratail-vein injection and were subsequently divided into 6 groups. At the third day after infection, the mice were treated with saline (group A), vector pVAX1 (greup B), rifampin (group C), vaccae (group D), Ag85A plasmid DNA vaccines (group E),rifampin and Ag85A plasmid DNA vaccines (group F) for 60 d. The lungs and spleens from the mice were taken and their pathological changes, weight and number of myeobacterial colony were examined at the third week after the end of treatment. Results At third week af-ter the end of treatment, the gross pathological observation and histopathological examination in lung showed that the lung lesions were limited, the profile of the alveoli was relatively clear, and normal structure could be seen in 2/3 areas of the lung sections in group D, E and F. The extent of lung lesion was 50% in group D,20% in group E and F. The pathological changes in group A, B, and C were more severer than those in group D, E and F. Compared with group A, the colony-forming units (CFU) in the lungs from mice in group D,E and F decreased 52%, 68%, 78%, respectively. The CFU in the spleens from mice in group D,E and F decreased 48%, 65%, 79%, respectively. Conclusion Ag85A plasmid DNA vaccines alone or Ag85A plasmid DNA vaccines along with chemotherapy have significant therapeutic effects on the mouse model of MDR-Mycobacterium tuberculosis infection.
