Expression of costimulating factor B7-H4 in patients with primary billary cirrhosis
- VernacularTitle:原发性胆汁性肝硬化患者共刺激分子B7-H4的检测及临床意义
- Author:
Chen ZHAO
;
Yongzhe LI
;
Yang ZHAO
;
Chaojun HU
;
Dinghua LIU
;
Dawei TONG
;
Shulan ZHANG
- Publication Type:Journal Article
- Keywords:
Primary biliaty cirrhosis;
Peripheral blood mononuclear cells;
Costimulating factor;
B7-H4;
Anti-mitochondrial antibody
- From:
Chinese Journal of Microbiology and Immunology
2008;28(9):851-855
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the expression of eostimulating factor B7-H4 in patients with primary biliary cirrhosis (PBC) and the pathogenesis of PBC. Methods The expression of B7-H4 mRNA on peripheral blood mononuclear cell (PBMC) of 65 patients with PBC was tested by real-time PCR. Serum levels of IL-2 were assayed by ELISA. CD4+, CD8+ T lymphocytes expression level and B7-H4 expression rate before and after activation were measured by three-color flow cytometry (FCM). Re-sults (1) Expression of B7-H4 mRNA and BT-H4 percentage in PBC group were significantly lower than that in none-PBC group and healthy controls(P<0.01);(2)After 72 h activation, the percentage of CD4+, CD8+, and CD4+ CD8+T lymphoeytes and serum levels of IL-2 decreased (P<0.05), and the percentage of CD4+, CD4+ CD8+T lymphocytes and serum levels of IL-2 were significantly higher than that of none-PBC group and healthy controls(P<0.01);(3)Levels of alanine aminotransferase(ALT), aspartate amin-otransferase(AST), alkaline phosphatase(ALP) and γ-glutamyl transpeptidase(GGT) of patients with posi-tive anti-mitochondrial antibody(AMA)-M2 rose. There was no significant difference of B7-H4 expressions on T cells between patients either with or without AMA-M2 antibody. Conclusion The costimulating factor B7-H4 can express on T lymphocytes which is activated by phytohemagglatinin(PHA) aad plays a negative role on T cells responses.
