Transplantation of autologous bone marrow mesenchymal stem cells transfected by angiogenin gene in a porcine chromic ischemic heart model
- VernacularTitle:携带血管生成素的骨髓基质干细胞移植治疗缺血性心脏病
- Author:
Sengdong HUANG
;
Xiaohong LIU
;
Yang YUAN
;
Dejun GONG
;
Zhiyun XU
- Publication Type:Journal Article
- Keywords:
Myocardial ischemia;
Myeloid progenitor cells;
Angiopoietins;
Mesenchymal stem cell transpantionTnafection
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2008;24(6):396-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the transplantation of autologous marrow mesenchymal stem cells transfected by angiogenin gene in a porcine chronic ischemic beart model.Methods Methods Mesenchymal stem cells were trnsfected byAd/Ang.The pigs underwent placement of amerold occluder around LCx and 4 weeks later sujected to transplantation of the trandected mesenchymal stem cells.The animals were evaluated by coronary angiography,echocardiography,mannetic resonance imaging end pathologic observation.Results All animal showed 95%occlusion fo LCx.4 weeks after treatment ,the perfusion of LCx,left ventricular ejection fracton were greatly evhanced.A large number of labeled mesenchymal stem cells were successfully uncorporated into blood vessels in the ischemic myocardial regions with increased vessels countin and survival of implanted cell.Conclusion Transplantaton of autologous mesenchymal stem cells trendected by angiogenin gene offere obvious advsntases of great improvement of blood supply and the heart funcition.
