Pravastatin inhibits ossific calcification of human umbilical artery vascular smooth muscle cells induced by tumor necrosis factor α
- VernacularTitle:普伐他汀抑制肿瘤坏死因子α介导的人脐动脉血管平滑肌细胞成骨样钙化
- Author:
Zhenyong LI
;
Zhaohui NI
;
Jiaqi QIAN
;
Huili DAI
;
Leyi GU
;
Yongping GUO
;
Mingshu SUN
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-alpha;
Pravastatin;
Calcinosis;
Alkaline phosphatase;
Human umbilical artery smooth muscle cell;
Ossific calcification
- From:
Chinese Journal of Nephrology
2008;24(12):915-919
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of pravastation intervention on tumor necrosis factor (TNF)-α-indueed ossifie calcification in human umbilical artery smooth muscle cells (hUASMCs). MethodshUASMCs were cultured by tissue explant in vitro, hUASMC were treated with TNF-α 50 μg/L and pravastatin of three different concentrations. The calcium deposition was determined by O-cresolphthalein eomplexone method. The mRNA expression of BAP and OPN was determined by real time-PCR. The protein expression of BAP, OPN and BMP-2 was determined by Western blotting. ResultsPravastatin inhibited the proliferation of hUASMC (r=-0.946, P＜0.01) and decreased the cell calcium deposition (r=-0.973, P＜0.01) in a dosedependent manner. Pravastatin down-regulated the expression of BAP, OPN and BMP-2 induced by TNF-α in a dose-dependent manner (mRNA, r=-0.972, P＜0.01;BAP protein, r=-0.820, P＜0.01;OPN protein, r=-0.972, P＜0.01;BMP-2 protein, r=-0.928, P＜0.01). ConclusionPravastatin can inhibit the proliferation of hUASMC, decrease the cell calcium deposition and inhibit the ossifie calcification of hUASMC induced by TNF-α.
