Endoscopic ultrasound guided 125Ⅰ seeds placement for celiac ganglion brachytherapy in porcine models
- VernacularTitle:家猪EUS引导下125Ⅰ放射性粒子腹腔神经节照射的损伤效果评价
- Author:
Kaixuan WANG
;
Zhendong JIN
;
Xianbao ZHAN
;
Jie CHEN
;
Zhaoshen LI
;
Yan LIU
- Publication Type:Journal Article
- Keywords:
Endoscopic ultrasonography;
Iodine radioisotopes;
Brachytherapy
- From:
Chinese Journal of Digestive Endoscopy
2008;25(12):635-638
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the influence of brachytherapy with 125Ⅰ seeds on celiac ganglia in porcine models. Methods Twelve pigs were randomly assigned into 3 groups to accept celiac plexus block by bilateral injection with 2 non-radioactive seeds in group A (n = 4), 0.4 mCi seeds in Group B (n = 4) and 0.8 mCi seeds in Group C (n = 4), respectively. Prophylactic antibiotics were administered postoperatively. Enhanced CT and three-dimensional reconstruction of blood vessels were performed to confirm the proper placement of the seeds. The animals were sacrificed 14 days and 60 days after the procedure, and TUNEL assay was employed to study neuron apoptosis in ediac ganglia. Results The procedure was succossfully performed in,10 pigs(83.3%)and failed in two others. The rescue procedure was performed on the day after and succeeded in both pigs. Apoptosis of neurons significantly increased in brachytherapy groups than in the control group and it was positively correlated with dose and time of radiation. Conclusion Brachytherapy can cause apoptosis of neurons of celiac ganglion, which could be the basis of its clinical application.
