The relationship between genotype of mouse and the immune response of hepatitis B vaccine
- VernacularTitle:小鼠不同基因型与乙肝疫苗免疫应答的关系研究
- Author:
Liying MA
;
Xi ZHONG
;
Bingfei YUE
;
Zhengming HE
;
Shuanghuan LIU
- Publication Type:Journal Article
- Keywords:
Hepatitis B vaccine;
Immune responses;
H-2;
NIH mouse
- From:
Chinese Journal of Microbiology and Immunology
2008;28(11):1020-1023
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the potency of hepatitis B vaccine in China. Methods Two inbred strains(DBA/1 and BALB/c) and two NIH closes-colonies of mice were typed in the H-2 region by microcy-totoxicity method and PCR. Groups of mice of the tested strains were immunized with the same hepatitis B vaccine, the titre of anti-HBsAg antibody was analyzed by microplate, and the ED50 was then estimated by Karder method for each strain. Results Significant differences were found between potency estimates de-rived from assays using different strains of mice. Conclusion It is likely that the variation of immune re-sponse to hepatitis B vaccine in mice is correlative with the H-2 haplotype. In some special case, the bet-erozygosity in H-2 region found in NIH stock could influence the accuracy in such testing even a reference preparation of hepatitis B vaccine was used. Base on our experiment, to select an appropriate NIH stocks with the H-2q haplotype for potency testing of hepatitis B vaccine in China.
