Effects of thalidomide on proliferation of human renal cancer cell and expression of basic fibroblast growth factor
- VernacularTitle:沙利度胺对人肾癌细胞增生及碱性成纤维细胞生长因子表达的影响
- Author:
Wenhua JIANG
;
Pengfei LIU
;
Xiaodong LI
- Publication Type:Journal Article
- Keywords:
Cell lines,tumor;
Fibroblast growth factors;
Renal neoplasms;
Thalidomide
- From:
Cancer Research and Clinic
2008;20(11):724-726
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the effects of thalidomide on proliferation of human renal cell line 786-0 and the expression of basic fibroblast growth factor (bFGF) in this cell line. Methods Cell was treated with different doses of thalidomide(6.25 μg/ml, 25 μg/ml, 100 μg/ml) respectively or normal saline as control; cell survival rate was analyzed by MTT assay. The mRNA level of bFGF was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). bFGF protein expression in 786-0 cells was detected by flow cytometry (FCM). Results Thalidomide, ranging from 6.25 μg/ml to 100 μg/ml, suppressed the proliferation of 786-0 cell line in vitro significantly. After application of thalidomide for 48 and 72 hours, the IC50 was 46.42 μg/ml and 19.56 μg/ml respectively. Apoptosis rate increased from 12.43 % to 30.30 %, accompanying with reducing expression of bFGF. Application of thalidomide (25 μg/ml) induced the most significant inhibition to the bFGF in the cell line. Conclusion Thalidomide down-regulates bFGF expression, inhibit the proliferation, and induce apoptosis in 786-0 cell line.
