Small interfering RNA targeting Rac1 gene inhibiting rat retinal neovascularization
- VernacularTitle:RNA干扰Ras相关C3肉毒素底物抑制大鼠视网膜新生血管生成
- Author:
Juanjuan LI
;
Meixia ZHANG
- Publication Type:Journal Article
- Keywords:
Retinal neovaseularization;
Genes,ras;
rac1 GTP-binding protein;
RNA,smallinterfering;
Animal experimentation
- From:
Chinese Journal of Ocular Fundus Diseases
2008;24(6):436-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the inhibited effects of small interfering RNA targeting Rac1(Rac1-siRNA)on rat retinal neovascularization in retinae.Methods Retinal vein occlusion was indueedby retinal photodynamic medthod in 25 Sprague-Dawley rats.Rae1-siRNA vector DNA was injected intothe vitrous of one eye of those rats(gene intervention group),and empty vector DNA was injected into thefellow eye(blank control group).Rae1-siRNA vector was injected in other 25 SD rats without retinal veinocclusion(blank intervention group).Two weeks after injection,fluorescein isothiocyanate(FITC)-dextran was perfused into the hearts of all the rats,and the retinal wholemount was made to observe theneovascularization.The numbers of endothelial cells which break through the internal limiting membranewere counted after hematoxylin-eosin staining.Resuits A massive of neovascularization and FITCleakage were found in blank control group.Small part of neovaseularization and a little FITC leakage wereobserved in the gene intervention group.Retinal vessels were normal in blank intervention group.Compared with blank contrast group and blank intervention group.the difference of the mean numbers ofendothelial cells which broke through the internal limiting membrane in the gene intervention group wassignificant(F=47.168,P=0.000).Conclusion Racl-siRNA can inhibit retinal neovascularizationinduced by retinal vein occlusion in rats.
