Establishment of platelet antigen panel and its application in the identification of platelet specific antibodies
10.3760/cma.j.issn.1009-9158.2009.02.010
- VernacularTitle:血小板谱抗原的建立及其在鉴定血小板特异性抗体中的应用
- Author:
Mingliang FENG
;
Wei SHEN
;
Zhonghui GUO
;
Tong SHEN
;
Biao YIN
;
Jianlian WANG
;
Sha JIN
;
Dazhuang LIU
- Publication Type:Journal Article
- Keywords:
Antigens,human platelet;
Antibody specificity
- From:
Chinese Journal of Laboratory Medicine
2009;32(2):162-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the platelet antigen panel for identifying the specificity of platelet antibodies which cause platelet transfusion refractoriness and neonatal alloimmune thrombocytopenia and provide evidence for clinical therapy and platelet genotyping research.Methods Based on the frequency distribution of human platelet alloantigen (HPA)-1 to HPA-16 gene in China, the frequencies of HPA-1 to HPA-6,HPA-15 alleles in blood group O donors were genotyped by the polymerase chain reaction with sequence-specific primers (PCR-SSP) method, and suitable donors were chosen to establish platelet-specific antigen panel.Using the established platelet-specific antigen panel, the specificity of platelet antibodies caused by alloimmune reaction was identified by using simplified sensitized erythrocyte platelet serology assay (SEPSA).Results Eleven ptatelet donors with blood group O were chosen to establish platelet-specific antigen panel which can identify specificity of HPA-1 to HPA-6, HPA-15 antibodies.One case of HPA-4b (Penb) and two cases of HPA-15a (Govb) platelet specific antibodies were detected in 1 120 samples.Conclusion Identifying the specific platelet antibodies using platelet specific antigen panel has profound significance on increasing the safety and effectiveness of clinical platelet transfusion and prevention of neonatal alloimmune thrombocytopenia.
