Effects of epigallocatechin gallate on reactive oxygen species in mouse podocytes exposed to high glucose
10.3760/cma.j.issn.1001-7097.2009.01.007
- VernacularTitle:表没食子儿茶素没食子酸酯对高糖环境下体外小鼠足细胞活性氧的影响
- Author:
Yan DAI
;
Qing YU
;
Qi XU
;
Jian YAO
;
Weijie YUAN
- Publication Type:Journal Article
- Keywords:
Podocytes;
Oxidative stress;
Reactive oxygen species;
Epigallocatechin gallate
- From:
Chinese Journal of Nephrology
2009;25(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of epigallocatechin gallate ( EGCG)on reactive oxygen species (ROS) in mouse podocytes exposed to high glucose. Methods Mouse podocytes cultured in high glucose were exposed to different concentrations of EGCG (0.2, 10, 100 μmol/L) or α-tocopherol (0.2 μmol/L) for 6, 12, 24 hours. The viability of podocytes was detected by MTT. The intracellular formation of ROS was detected by confocal microscopy with fluorescent probe CM-H2DCFDA and was measured by fluorescence microscopy. RT-PCR was used to examine the expression of p22phox, p47phox and p67phox mRNA in cultured podocytes exposed to different concentrations of EGCG. Results Intracellular ROS generation was significantly higher in high glucose than that in control conditions (P<0.01). EGCG could significantly inhibit ROS induced by high glucose significantly (P<0.01). EGCG (100 μmol/L) led to an inhibition of the increased production of NADPH oxidase components of p22phox and p67phox mRNA in high glucose (P<0.05). The expression of p47phox mRNA in high glucose was inhibited by EGCG(0.2 μmol/L) and ot-tocopherol(0.2 μmol/L) (P<0.05). Conclusion EGCG can protect cultured mouse podocytes from injury of high glucose by inhibiting ROS formation.
