Application of heterozygous ambiguity resolution primers resolving ambiguous genotyping results of human leukocyte antigen genes
10.3760/cma.j.issn.1009-9158.2009.01.009
- VernacularTitle:杂合性歧义引物分离法在解决人类白细胞抗原基因歧义分型结果中的应用
- Author:
Lianghong CHENG
;
Hongyan ZOU
;
Shizheng JIN
;
Zhen LI
;
Daming WANG
;
Xi CHENG
;
Yan JIANG
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
HLA antigens;
Genotype;
Base sequence;
Heterozygous ambiguity resolution primers
- From:
Chinese Journal of Laboratory Medicine
2009;32(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the heterozygous ambiguity resolution primers (HARPs) method in resolving ambiguous genotyping results of human leukocyte antigen (HLA) genes in Chinese Hart population, and choose some appropriate HARPs primers. Methods HLA-A, HLA-B and HLA-DRB1 genes of 416 southern Chinese Han individuals were genotyped by sequence-based-typing(SBT) method and then the ambiguous genotyping samples were sequenced again by HARPs primers provided by American Atria company. Results The percentage of ambiguous genotyping samples resolved by HARPs for HLA-A, HLA-B and HLA-DRBI locus was 86.3% (132/153), 73.9% (130/176) and 38.1% (85/223) respectively. Among them, 48.5% (64/132)HLA-A, 80.0% (104/130)HLA-B and all HLA-DRB1(85/85)samples only need one primer, 47.7 % (63/132)HLA-A and 20.0% (26/130)HLA-B samples need two primers. Three to six different HARPs primers can resolve more than 90% ambiguities. Conclusion HARPs is a convenient method and could be a routine method to resolve ambiguities for HLA-A, HLA-B and HLA-DRB1 genes genotyped by SBT in Chinese Han population.
