Establishment of the national standard materials for haemiglobincyanide
10.3760/cma.j.issn.1009-9158.2009.01.026
- VernacularTitle:氰化高铁血红蛋白国家一级标准物质的研制
- Author:
Mingting PENG
;
Chengjin AI
;
Chenbin LI
;
Xiaolin GU
- Publication Type:Journal Article
- Keywords:
Themoglobins;
Reference standards;
Spectrophotometry
- From:
Chinese Journal of Laboratory Medicine
2009;32(1):97-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the national standard materials for haemiglobincyanide (HiCN) for the traceability assays of hemoglobin. Methods HiCN national standard materials were established according to the document of International Committee for Standardization in Haematology (ICSH). The standard materials were certificated according to ISO Guide 35, including homogeneity and stability. Then they were characterized by the calibrated spectrophotometer which can be traceable to National Institute of Standards and Technology (NIST). The international reference materials of HiCN were compared with the result of the WHO reference laboratory to confirm the reliability. Results The uncertainty of the HiCN standard materials was 0.000 4 g/L and the variation coefficient (CV) was 0.09%. The uncertainty of long-term stability was 0.000 6 g/L; the certificated value of the standard materials was 0.615 9 g/L with uncertainty of 0.000 4 g/L. The combined uncertainty was 0.000 9 g/L and the expanded uncertainty was 0.001 8 g/L when the cover factor was 2. The relative error was 0.08% between the result of the standard materials and the international certificated value. Conclusion The homogeneity and stability of the standard material is acceptable and the method of characterization is accurate and reliable.
