Role of high mobility group box chromosomal protein 1 in pathogenesis of ulcerative colitis in mice
10.3760/cma.j.issn.1007-5232.2009.01.011
- VernacularTitle:高迁移率族蛋白-1在小鼠溃疡性结肠炎发病中的作用研究
- Author:
Zonghai LU
;
Lin LIN
;
Lei CHEN
;
Hui LI
;
Ruihua SHI
;
Xueliang LI
- Publication Type:Journal Article
- Keywords:
Colitis,ulcerative;
High mobility group box chromosomal protein 1;
Mice;
Sodium dextran suifate
- From:
Chinese Journal of Digestive Endoscopy
2009;26(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish ulcerative colitis(UC)model in BALB/c mice and to investigate the role of high mobility group box chromosomal protein 1(HMGBI)in pathogenesis of UC.Methods Thirty-two BALB/c mice were randomly divided into UC group(n=24,which were fed with 3%dextran sulfate 80dium solution)and control group(n=8,which were fed with water).The animals were sacrificed at 24.96 and 1 68 hours,respectively,to collect samples of colon and blood.The sernm level of HMGB1 was measured with ELISA and the expression of HMGB1 in colon was determined by Western blotting analysis.Results Histological scoring increased with the induction of the model,and manifestation of colon mucosa at 168h was similar with that of UC in human.The serum level of HMGB1 was slightly higer at 24 h than that of control(5.09±0.61 μg/L vs 4.49±0.53μg/L,P＞0.05),and reached a peak at 96 h (14.74±0.60 μg/L,P＜0.01),decreased at 168 h(9.03±0.78μg/L,P＜0.01).The expression 0.05).significandy increased at 96h(0.76±0.03,P＜0.05)and at 168 h(0.77±0.04,P＜0.05).Conclusion HMGB1 might be involved in pathologic changes of UC at a later stage.
