Evaluation of the value of an automatic bacteria identification system and blaOXA-51-like gene amplification in the identification of acinetobacter species
10.3760/cma.j.issn.1009-9158.2009.01.022
- VernacularTitle:自动化细菌鉴定仪和碳青霉烯酶基因OXA-51扩增在不动杆菌属细菌鉴定中的价值
- Author:
He WANG
;
Hui WANG
;
Yingchun XU
;
Minjun CHEN
- Publication Type:Journal Article
- Keywords:
Acinetobacter;
Bacterial proteins;
Beta-lactamases;
Palymorphism,restriction fragment length;
Polymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2009;32(1):83-87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of automatic bacteria identification systems including Phoenix and Vitek2 Compact, as well as blaOXA-51-like gene amplification in the identification of Acinetobacter species compared with amplified ribosomal DNA restriction analysis(ARDRA). Methods A total of 50 non-repeated clinical isolates of Acinetobacter species were collected in PUMCH from 2006 to 2007 and identified by ARDRA, blaOXA-51-like gene amplification, Phoenix and Vitek2 Compact,respectively. Results Compared with ARDRA, the sensitivity and specificity of blaOXA-51-like gene amplification were both 100%. The accuracy rates of Phoenix and Vitek2 Compact were 44% and 56%, respectively. Conclusions The accuracy of phenotypic identification of Acinetobacter species is not ideal, however, blOXA-51-like gene amplification could be used as a fast and reliable method for identification of A. baumannii. ARDRA is recommended in the study of drug resistant mechanisms and homological analysis of Acinetobacter species.