Immunological effect of tumor cell vaccines to hepatoma A (HepA) treated by different adjuvant of Haematoxylin
10.3760/cma.j.issn.1006-9801.2009.01.004
- VernacularTitle:苏木精修饰瘤苗加入不同佐剂对HepA的抑瘤作用
- Author:
Fang YANG
;
Cong ZHANG
;
Xihua BAI
;
Na ZHAO
;
Lei YAN
;
Yongming YANG
;
Liansheng REN
- Publication Type:Journal Article
- Keywords:
Haematoxyhn;
Cell line,tumor,Liver neoplasms,exprimental;
Immunization
- From:
Cancer Research and Clinic
2009;21(1):11-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the immunological effect of tumor cell vaccines to mouse hepatoma A(HepA) treated by Haematoxylin. Methods HepA cell was treated by 0.1% Haematoxylin and made into three vaccines: HepA vaccine with complete Freund' s adjuvant, HepA vaccine with incomplete Freund' s adjuvant; and HepA vaccine without any adjuvants. Five times of immunization were given the grouped mice with the above three vaccines, then active HepA cells (1×105 for each mouse) were inoculated by intraperitoneal injection to attack them; reckoning the mean survival time (MST) of the grouped mice, comparing the immunoprotective action of the three vaccines to the tmnor-bearing mice. Those mice only receiving normal saline (equal volume to the vaccine) were as a control group. Results MST of control group was (23.30±1.24) day; MST of mice receiving H22 vaccine with complete adjuvant was (43.90±15.20) day (P<0.02); MST of mice receiving H22 vaccine with incomplete adjuvant was (39.60±13.77) day (P<0.05); and MST of mice receiving HepA vaccine without any adjuvant was (38.40±12.54) day (P<0.05); As compared with the control group, the three treated groups showed a life-lengthening rate (LLR) 88.41%, 69.96 % and 64.81% respectively. Conclusion The vaccines treated by Haematoxylin give a marked immunoprotective action to those tumor-bearing mice. The HepA vaccine' s immunological effect is increased by the Freund' s adjuvant (complete or incomplete).
