Analysis of the resistance of Helicobacter pylori strains to clarithromycin in west-Guangxi region

VernacularTitle:桂西地区幽门螺杆菌对克拉霉素的耐药性分析
Author: Yanqiang HUANG ; Lijuan ZHAO ; Zansong HUANG ; Xiaohua LI ; Xihan ZHOU ; Chao CEN
Publication Type:Journal Article
Keywords: Helicobacter pylori; Drug-resistance; Clarithromycin
From: Chinese Journal of Microbiology and Immunology 2009;29(1):37-40
CountryChina
Language:Chinese
Abstract: objective To analyze the prevalence of Helicobacter pylori(Hp)strains resistant to clarithromycin and the relation of 23S rRNA genetic mutation with clarithromycin resistance.Methotis Hp strains were isolated from gastric mucosa biopsies of patients with peptic ulcer or gastritis.Resistanee of the isolates to elarithromycin wag determined using diffusion test.Mutations in elarithromyein resistant strains were identified by PCR-RFLP and gene sequencing.Results The prevalence of Hp strains resistant clarithromyein was 22.2%.10 of clarithromycin resistant strains had A2143G.A2144G point mutation in 23S rRNA gene,and none of the 10 susceptible strains had the A2143G or A2144G mutation,and the result was affirmed by measure sequencing.Conclusion The prevalence of Hp is higher than Beijing and Shanghai,mutations in 23S rRNA are associated with elarithromyein resistance in Hp.