Stability and reproducibility study in serum proteomics with matrix assisted Iaser desorption ionisation time-of-flight mass spectrometry

VernacularTitle:用基质辅助激光解吸电离飞行时间质谱进行血清蛋白质组学分析中样品稳定性研究
Author: Feng QIU ; Yaping TIAN
Publication Type:Journal Article
Keywords: Spectrometry,mass,matrix-assisted laser desorption-ionization; Proteomics; Reference values
From: Chinese Journal of Laboratory Medicine 2009;32(2):148-152
CountryChina
Language:Chinese
Abstract: Objective To study the stability and reproducibility in serum proteome by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).Methods Bruker Daltonics MALDI-TOF-MS was adopted under the linear mode, with mass ranging from 800 to 10 000 m/z.MB-WCX pre-extraction kit and Clinprotools software was used to analyze data.Results The reproducibility study was carried out under the standardized conditions.Data analysis showed that the m/z ratio was in the acceptable range, and the coefficients of variation(CV) was 12.7% ,13.1% and 18.8% ,respectively in the parallel experiment by taking 2 cancer and 1 healthy control serum samples and could identify the diseased samples.Then 30 healthy control samples were used to validate this result, all of which showed very good mass profile. The average CV was 25.5%, which could meet the requirements of clinical laboratory.Conclusion With the help of optimized experimental conditions and MALDI-TOF-MS, stable and reproducible mass spectrum can be required which is helpful for the early diagnosis of diseases.