FTY720 induces apoptosis of rat glomerular mesangial cells
10.3760/cma.j.issn.1001-7097.2009.01.010
- VernacularTitle:FTY720诱导大鼠系膜细胞的凋亡
- Author:
Mei CHEN
;
Jingyu JIANG
;
Yu ZHANG
;
Yi WANG
;
Yan HAO
;
Jianhua ZHOU
- Publication Type:Journal Article
- Keywords:
Cell proloferation;
Apoptosis;
Cyclins;
Mesangial cells;
Oligonucleotide Array Sequence Analysis;
FTY720
- From:
Chinese Journal of Nephrology
2009;25(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of a new immunosuppressive agent, FFY720, on rat glomerular mesangial cell (GMC) apoptosis and on gene expression profiles of cell cycle regulatory proteins. Methods Rat GMCs were cultured with 20 μmol/L FTY720 for 6 h, 12 h, 24 h and 48 h, and then were evaluated for proliferation through MTT method, and for apoptosis by flow cytometry and fluorescence stainig with Hoechst33258 and PI, and DNA fragmentation analysis. The gene expression profile of cell cycle regulatory proteins was characterized in rat GMCs before and after FTY720 treatment by SuperArray real-time PCR microarray analysis. Results After incubation with FTY720 for 6 h, apoptotic sub-G1 peak was identified in GMC through flow cytometry. After incubation with FTY720 for 12 h, not only apoptosis bodies of GMC were observed by fluorescence staining with Hoechst33258 and PI, but also typical morphological changes of apoptosis were found in GMC. After incubation with FRY720 for 24 h, typical DNA ladder pattern was identified. The percentage of FTY720-iuduced GMC apoptosis gradually increased with the extension of incubation time. SuperArray real-time PCRmicroarray analysis revealed that FTY720 could respectively up-regulate the expression of Dnajc2, LOC688900 and RGDi562436_predicted genes to 41.6, 38 and 16 folds. Conclusion FTY720 can induce GMC apoptosis in a time-dependent manner, probably through influencing gene expression of cell cycle regulatory proteins.
