Expression of Ang-1, Ang-2 and Tie-2 receptor mRNAs and their implications in hemangioma by RT-PCR

VernacularTitle:反转录-聚合酶链式反应检测促血管生成素-1和促血管生成素-2及Tie-2受体的mRNA在血管瘤组织中的表达及其意义
Author: Fan HONG ; Fulian ZHUANG ; Biao WANG ; Jingan LIN
Publication Type:Journal Article
Keywords: Hemangiomas; Angiopoietin; Tie-2 reeeptor; Cell proliferation associated pro-tein Ki-67
From: Chinese Journal of Medical Aesthetics and Cosmetology 2009;15(1):49-52
CountryChina
Language:Chinese
Abstract: Objective To detect the expression level of Ang-1, Ang -2 and Tie-2 receptor mRNAs in the hemangioma tissuses and to explore the effects of Ang-1, Ang-2, and Tie-2 receptor on the angio-genesis in the hemangioma. Methods By use of semiquantinative reverse transcription polymerase chain reaction, we detected the expression level of Ang-1 mRNA, Ang-2 mRNA and Tie-2 mRNA in 30 cases of hemangioma (17 specimens of proliferating hemangioma, and 13 involuting hemangioma )together with 10 cases of normal skin as control, and the expression level of Ki-67 protein was also detected by an im-munohistochemica method. Results The expression of Ang-2 mRNA and Tie-2 mRNA in the prolifera-ting hemangiomas was higher than that in involuting hemangiomas (P<0.01) . The expression of Ang-1 mRNA in hemangiomas was negative. Conclusion The unbalance of Ang-Tie-2 system is probably impor-tant for the pathogenesis and development in hemangiomas.