Lactobacillus acidophilus inhibits expression of IL-6 and IL-8 in Caco-2 cells induced by TNF-α
10.3760/cma.j.issn.0254-5101.2009.01.008
- VernacularTitle:乳杆菌对TNF-α诱导Caco-2细胞分泌IL-6和IL-8的影响
- Author:
Bin WANG
;
Qiurong LI
;
Qian HUANG
;
Ning LI
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Lactobacillus acidophilus;
TNF-α;
Caco-2;
IL-6;
IL-8
- From:
Chinese Journal of Microbiology and Immunology
2009;29(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of live Lactobacillus acidophilus on Caco-2 cells to release pro-inflammatory cytokines IL-6 and IL-8.Methods Caco-2 cells were cultured for 2 h with live Lactobacillus acidophilus strain 1950103-12 and examined by reverse transcription-PCR for IL-6 and TNF-α induced IL-8 expression.An enzyme-linked immunosorbent assay was used to quantitate secreted IL-6 and IL-8.Results Lactobacillus acidophilus strain 1950103-12 had no ecffect on the mRNA expression and production of IL-6 and IL-8 in the Caco-2 cells.A significant mRNA expression and production of IL-6 and IL-8 by the Caco-2 cells were detected after exposure to TNF-α.Lactobacillus acidophilus strain 1950103-12 down-regulated IL-6 and IL-8 mRNA expression and inhibited constitutive synthesis by Caco-2 cells of IL-6 and IL-8 that induced by TNF-α.Conclusion Lactobacillus acidophilus strain 1950103-12 has potent direct anti-inflammatory activity on human epithelial cells.
