Fiber genetic characteristics of 7 human adenovirus isolates
10.3760/cma.j.issn.0254-5101.2009.01.015
- VernacularTitle:7株B亚属人腺病毒纤维基因特征分析
- Author:
Liuying TANG
;
Lili ZHANG
;
Zhen ZHU
;
Wenbo XU
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Fiber gene;
Genetic characteristics
- From:
Chinese Journal of Microbiology and Immunology
2009;29(1):62-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genotypes of adenovirus(Ad)strains isolated from young children with acute respiratory infection syndrome during 2004-2006.Methods Seven human adenovirus strains isolated during 2004 and 2006 were detected with tissue culture,PCR with adenovims specific-primers and sequencing for hexon and fiber genes.Results The results showed that all 7 adenovirus strain belonged to adenovirus genera B.3 of the 7 isolates were Ad3,BJ060605-1,BJ060316-and BJ040228 had high homology of nucleic acid.98.9%-100%.Three of 7 were Ad7,the homology of nucleic acid of BJ050520.BJ060421-4 and BJ040226 were 97.6%-100%.Strain SX060418-1 was 100% homologic with Ad11 strain L08232(GenBank).Conclusion Little variation of fiber gene was found for 2004 to 2006 isolares and the typing with fiber sequencing is similar with that of hexon gene of adenovirus isolates.