Screening for nasopharyngeal carcinoma antigens that induce humoral immune response by serological proteomics analysis

Bin YI; Ying Chen; Cui LI; Pengfei Zhang; Maoyu LI; Hong YI; Fang Peng; Zhuchu Chen; Zhiqiang Xiao

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Screening for nasopharyngeal carcinoma antigens that induce humoral immune response by serological proteomics analysis

VernacularTitle:采用血清蛋白质组学方法筛选能诱发自身抗体的鼻咽癌相关抗原
Author: Bin YI ; Ying CHEN ; Cui LI ; Pengfei ZHANG ; Maoyu LI ; Hong YI ; Fang PENG ; Zhuchu CHEN ; Zhiqiang XIAO
Publication Type:Journal Article
Keywords: Nasopharyngeal neoplasms; Proteomies; Antigens,neoplasm; Autoantibodies; Tumor markers,biological
From: Chinese Journal of Laboratory Medicine 2009;32(2):142-147
CountryChina
Language:Chinese
Abstract: Objective To identify proteins eliciting a humoral immune response in patients with nasopharyngeal carcinoma (NPC) by a serological proteome analysis, and provide candidate biomarkers for diagnosis and treatment of NPC.Methods Two-dimensional (2-DE) electropboresis was used to separate total cellular proteins from 19 NPC tissues.Separated proteins were transferred onto PVDF membranes and sera from 19 NPC patients and 19 healthy subjects were individually screened by western blotting for antibodies that react against separated proteins.Each tissue samples was subjected to three 2-DE gels and coomassie staining was performed in one of them.The protein spots which selectively reacted with the patient sera were excised from the preparative gels and subjected to further analysis of MOLDI-TOF MS and ESI-QTOF MS/MS.The proteins were identified based on peptide mass fingerprints and peptide sequence tags followed by searching database.Results In this study, 13 NPC associated antigens (HSP 70, HAS,HSP 60, CK 15, LAP 3, α-enolase, EBP 1, CK 19, ribosomal protein P 0, pyrovate dehydrogenase E1,guanine nucleotide-binding protein, prohibitin, Rho-GDI 2) that elicited an antibody response in most of NTC patients were identified.The positivities of these proteins were more than 21% all in NPC patients, but were lower or even absent in normal subjects.Conclusion These 13 NPC associated antigens and their autoantibodies may be useful for NPC diagnosis and treatment.