Determination of sodium in serum by inductively coupled plasma mass spectrometry
10.3760/cma.j.issn.1009-9158.2009.01.025
- VernacularTitle:电感耦合等离子体质谱测定血清钠
- Author:
Ying YAN
;
Yueqin LIANG
;
Shui YU
;
Yaping TIAN
;
Chuanbao ZHANG
;
Wenxiang CHEN
;
Ziyu SHEN
- Publication Type:Journal Article
- Keywords:
Sodium;
Mass spectrometry;
Serum
- From:
Chinese Journal of Laboratory Medicine
2009;32(1):92-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a candidate reference method for the determination of sodium in serum by inductively coupled plasma mass spectrometry method (ICP-MS). Methods Aluminum, as internal standard of sodium, was added into serum samples and sodium standard solutions by gravimetric analysis. The samples were digested by HNO3 and diluted, and its 23Na/27Al isotope ratios were obtained by ICP-MS. The sodium concentrations were calculated with the standard curve method in serum. Results The analytical recoveries of sodium were 100.67% and 100.15% respectively, and the precisions were 0.08% and 0.04% respectively for 2 different serum samples. The results of measuring sodium in serum of Standard Reference Material (SRM) gave the coefficients of variation (CVs) of 0.18% and 0.22% for 2 levels of standard reference material(SRM) 909b and 0.41%, 0.41% and 0.66% for 3 levels of SRM 956b. The relative deviations between the results and median of the certified value were 0.17% and 0.14% for 909b and -0.09%, -1.05%, -0.48% for 956b respectively. Conclusions The ICP-MS and aluminum internal standard method is proved to be not only precise and accurate, but also quick and convenient for measuring sodium in serum. It is promising to be a candidate reference method for determination of sodium in serum.
