The effect of respiratory syncytial virus infection on neutrophil adherence to airway epithelial cells.
- Author:
Hyun Hee KIM
;
Joon Sung LEE
;
Sung Hoon CHO
- Publication Type:Original Article
- Keywords:
BEAS-2B cell line;
IL-8;
ICAM-1;
respiratory syncytial virus;
neutrophil adherence
- MeSH:
Cell Culture Techniques;
Child;
Epithelial Cells*;
Gene Expression;
Humans;
Infant;
Inflammation;
Intercellular Adhesion Molecule-1;
Interleukin-8;
Neutrophils*;
Peroxidase;
Respiratory Syncytial Viruses*;
RNA, Messenger
- From:Journal of Asthma, Allergy and Clinical Immunology
1999;19(1):50-61
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Respiratory syncytial virus (RSV) is the most common cause of lower respiratory infection in infants and young children, but the pathogenesis of RSV-induced inflammation is not well defined. MATERIAL AND METHOD: In order to examine the potential interactions between virus-infected airway epithelial cells and neutrophils, we studied the ability of neutrophils to adhere to yirus-infected airway epithelial cell monolayers by myeloperoxidase assay. Also we measured the ability of airway epithelial cells to secrete interleukin-8(IL-8) and inter-cellular adhesion molecule-1(ICAM-1) in virus-infected airway epithelial cell cultures by enzyme-linked immunosorbent assay(ELISA). The degree of IL-8 and ICAM-1 gene expression in the RSV-infected BEAS-2B cell cultures were analyzed by reverse transcription-polymerase chain reaction(RT-PCR). RESULTS: The RSV-infected BEAS-2B cell resulted in significantly enhanced level of neutrophil adherence compared to the uninfected control(p (0.001). IL-8 and ICAM-1 production significantly increased by RSV infection(p<0.05). There was a significant positive correlation between neutrophil adherence and IL-8 level(r=0.73, p=0.002), and ICAM-1 level (r=0.843, p=0.001) in RSV-infected cells. The degree of both IL-8 and ICAM-1 mRNA expression increased in the RSV-infected cells compared with the uninfected ones. CONCLUSION: These results suggest that RSV infection significantly enhances the production of IL-8 and ICAM-1 in airway epithelial cells which then results in increased neutrophil adherence.
