Discovery of biomarkers from serum in hepatocellular carcinoma using protein chip technique
10.3760/cma.j.issn.1006-9801.2009.01.006
- VernacularTitle:蛋白芯片技术对甲胎蛋白阴性肝癌诊断的意义
- Author:
Yunbin YE
;
Huijing CHEN
;
Dong ZHOU
;
Mingzhi HAO
;
Fang LIU
;
Jiami YU
;
Xing LI
;
Hailan LIN
;
Qiang CHEN
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocelhlar;
Tumor markers,biological;
SELDI;
Alpha-fetoproteins
- From:
Cancer Research and Clinic
2009;21(1):17-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen serum biomarkers in patients with hepatocelhlar carcinoma by using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry(SELDI-TOF-MS)technique and to evaluate its clinical implication in the patients whose alpha-fetoprotein were negative in the sera. Methods Proteomic spectra were generated by mass spectroscopy in 112 cases, including 57 cases AFP-negative hepatocellular carcinoma,and 55 cases of healthy control. The consequence was analyzed and the characteristic preteomic peaks was selected by using Biomarker Wizard. Results Seven low expressed potential biomarker were indentified with the mass-to-charge ratio of 4.2×103, 4.1×103, 6.7×103, 5.7×103, 6.5×103, 6.9×103, 5.8×103. The sensitivity for diagnosing hepatic cancer were 88.23 % and specificity was 92.31%. These peaks were not correlated with age, sex, tumor mass size, pathology grading and cirrhosis in hepatocellular carcinoma. Conclusion SELDI-TOF-MS offers a unique platform for the proteomic detection of hepatocellular carcinoma.It also offers an auxiliary diagnosis method to the patients whose alpha-fetoprotein are negative in the serum.
