Galectin-9(Tim-3L)significantly attenuates allogeneic immune response in mice
10.3760/cma.j.issn.0254-5101.2009.01.002
- VernacularTitle:Galectin-9(Tim-3L)显著减弱小鼠移植排斥
- Author:
Wentao HE
;
Jing YUAN
;
Zemin FANG
;
Feng WANG
;
Yi XU
;
Hongmin ZHOU
;
Ying GAO
;
Weina ZHANG
;
Lu WANG
;
Zhonghua CHEN
- Publication Type:Journal Article
- Keywords:
Tim-3;
Galectin-9;
Immunosuppression;
Mouse
- From:
Chinese Journal of Microbiology and Immunology
2009;29(1):5-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the subcellular localization of Galectin-9 and its effect on allogeneic immune response.Methods The plasmid pEGFP-N1 was inserted with Galectin-9 fragment which was amplified from pBKCMV-Galectin-9 by PCR.The recombinant plagmid wag then transfected into CHO cells using JetPEI in vitro.The cells were cultured in G418 selecting mediam to obtain the stably-transfected cells.The transcription and expression of Galectin-9 gene were verified by immunohistochemical staining and RT-PCR.The solid-phase transgenic CHO cells or freshly-cultured supernatant wag added into the mixed lymphocyte response system to detect the inhibitory effect of Galectin-9.Galectin-9 protein wag administered intraperitoneally for 7d consecutively.Results The expression of Galectin-9 wag localized in the cytosol of CHO.The allogeneic mix lymphocyte proliferation was dose-dependently inhibited by the freshly-cultured supernatant from stably-transfected CHO cells.Furthermore,the supernatant from stably-transfected CHO cells dose-dependently inhibited the level of IL-2.The inhibitory effect could be reversed by Tim-3-Fc blocking.Administration of Galectin-9 significantly prolonged the survival of allogeneic cardiac transplants[(22.7±1.2)d vs(7.2±0.4)d)].Conclusion Galectin-9 may be secreted in physical situation to exert its immunomodulatory function on allogeneic immune response.Furthermore.Galectin-9 may be a novel therapeutic drug in transplant medicine.
