The induction of CD8+CD28- regulatory T cells by donor specific transfusion and their effect on the acute rejection responses in rats liver transplantation

VernacularTitle:供体特异性输血诱导CD8+CD28-调节性T细胞在肝移植急性排斥反应中的作用
Author: Ning CHEN ; Guodong CHEN ; Peng YOU ; Yulan LIU
Publication Type:Journal Article
Keywords: Liver transplantation; CD8+CD28- regulatory T cells; Donor specific transfusion; Acute rejection response
From: Chinese Journal of Microbiology and Immunology 2009;29(2):142-145
CountryChina
Language:Chinese
Abstract: Objective To acquire antigen specific CD8+CD28-Tr by donor specific transfusion (DST) in vivo, and evaluate their suppressive effect on the acute rejection responses in rat liver transplantation through adoptive transfer experiment. Methods DST was used to induce CD8 + CD28 Tr to LEW antigens in naive Brown Norway(BN) rat. Then the induced CD8+CD28- Tr were adoptively transferred into the recipients of LEW→BN liver transplantation, which were acute rejection models. The survival time and histological changes were observed. Statistic analysis was performed with Log-Rank test by SPSS11.0 software, to compare the survival rate. Results Adoptive transfer of the DST-induced CD8+CD28-Tr attenuated the acute rejection responses in acute rejection models of rat liver transplantation. Conclusion DST can induce large numbers of CD8+CD28-Tr in naive BN rats in vivo. The adoptive transfer of the induced CD8+CD28Tr suppressed the acute rejection responses in rat liver transplantation. DST may become one of the methods that induce antigen specific CD8+CD28- Tr in vivo.