Reduced apoptosis induced by endotoxin in mice blocking TRAIL with soluble death receptor 5
10.3760/cma.j.issn.0254-5101.2009.02.013
- VernacularTitle:sDR5封闭TRAIL减少内毒素引起小鼠肝细胞凋亡
- Author:
Huiling BAI
;
Xueyin WANG
;
Shulian LI
;
Hongying HUANG
;
Yaowu DU
;
Guangchao LIU
;
Yuanfang MA
- Publication Type:Journal Article
- Keywords:
Endotoxin;
Lipopolysaccharides;
Soluble death receptor 5;
TNF related apoptosis inducing ligand;
Hepatocyte apoptosis
- From:
Chinese Journal of Microbiology and Immunology
2009;29(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of TNF related apoptosis inducing ligand (TRAIL) in apoptosis induced by LPS. Methods After LPS injected mice blocking TRAIL with soluble death receptor 5 (sDRS), detecting ALT, AST and LDH of mice serum at different times, apoptotic effects of LPS to mice hepatocyte were detected by HE and flow eytometry (FCM) with Annexin V-FITC/PI staining. The expres-sion of DR5 in mice hepatocyte was assayed with immunohistochemistry and FCM. Results Apoptotic effect was promoted by up-regulated DR5 expression on hepatocyte. Blocking TRAIL with sDR5 markedly amelio-rated the hepatocyte damage and reduced apoptosis. Conclusion These results establish a critical role for TRAIL in apoptosis during disease process of LPS.
