Detection of prototype foamy virus by loop-mediated isothermal amplification

VernacularTitle:环媒恒温扩增技术(LAMP)检测原型泡沫病毒
Author: Xiaobo BAI ; Bin YANG ; Qimin CHEN ; Yunqi GENG ; Wentao QIAO
Publication Type:Journal Article
Keywords: Prototype foamy virus; Loop-mediated isothermal amplification; Detection
From: Chinese Journal of Microbiology and Immunology 2009;29(2):181-185
CountryChina
Language:Chinese
Abstract: Objective To develop prototype foamy virus (PFV) detection method by loop-mediated isothermal amplification. Methods Three pairs of primers targeting core region of PFV integrase were designed in this study and Bst DNA polymerase was used to amplify target sequence at 63℃. The system was established with all the conditions optimized. Results The method was established with the plasmid containing target sequence as the template. This method could specifically detect PFV infectious clone, no crossreaction was observed with human immunodeficieney virus infectious clone, bovine immunodefieiency virus infectious clone and bovine foamy virus infectious clone as templates. The detection capability of this system was 50 copy, one order more sensitive than PCR. The amplification could be finished in 15 min and human genomic DNA did not adversely affect the amplification efficiency. Conclusion The PFV detection method by loop-mediated isothermal amplification was established and it had potential usefulness in PFV detection.