The hyper-variable region genotypes of methicillin-resistant Staphylococcus aureus and their relationship with the resistance
10.3760/cma.j.issn.0254-5101.2009.02.003
- VernacularTitle:耐甲氧西林金黄葡萄球菌高变区基因分型及其与耐药性的关系
- Author:
Min WANG
;
Xianping LI
;
Wenjuan LI
;
Bing HE
- Publication Type:Journal Article
- Keywords:
Methicillin-resistant Staphylococcus aureus;
Hyper-variable region;
Type;
Resistance
- From:
Chinese Journal of Microbiology and Immunology
2009;29(2):108-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the hyper-variable region-polymerase chain reaction(HVRPCR) genotype of methicillin-resistant Staphylococcus aureus (MRSA) in local hospitals in Hunan province, and to compare it with the antibiograms, and to preliminarily discuss its role in molecular epidemiology of MRSA. Methods A total of 80 MRSA clinical isolates were collected from three affiliated hospitals of Central South University. Their DNA were extracted and amplified by PCR. The genotype was classified by the fragments of amplified products based on the size of HVR. The drug sensitivity test of MRSA was performed, and the correlation of genotypes and antibacterial resistance was analyzed. Results Eighty strains of MRSA were divided into 5 HVR genotypes which were named as A, B, C, D and E respectively according to the size of the PCR products. The most common types were D (61.25%) and E (21.25%), followed by A (3.75%), B (5.00%) and C (8.75%). Most strains of genotypes were multi-drug resistant but all strains were sensitive to vancomycin. Conclusion These results suggest that HVR-PCR genotype method is a rapid, convenient and effective method for epidemiological investigation of infections caused by MRSA, and it is also helpful for clinical selection of antibacterial agents in effective treatment of MRSA infection.
